miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1

Su, Xiaoping; Qian, Cheng; Zhang, Qian; Hou, Jin; Gu, Yan; Han, Yanmei; Chen, Yongjian; Jiang, Minghong; Cao, Xuetao

To mutually corroborate the expression profiles obtained from in vitro DCs, CD11c þ Ia high cells (the in vivo counterpart of mature DCs), CD11c þ Ia low cells (the in vivo counterpart of regulatory DCs) from the murine splenocytes were sorted as described previously 15 . The total RNA was isolated and sequenced on Illumina platform. A total of 255 miRNAs (TPM410 in at least one small RNA library) were selected for further analysis. We analysed the expression profile alteration in mature DCs-regulatory DCs development in vitro and in vivo. About half of miRNAs (121/255, 47.5%) have the same alteration pattern. Among these miRNAs, 29 miRNAs such as miR-30b, let-7, miR-146a and miR-22 increased their expression, whereas 92 miRNAs such as miR-132, miR-126, miR-93 and miR-146b decreased their expression in this process both in vivo and in vitro (Fig. 1d) . So many miRNAs have same alteration pattern in vitro and in vivo, suggesting their specific biological functions in the process (maDCs-DCreg). All 255 miRNAs were listed in Supplementary Data 2.