Author: Kammer, Andreas R.; van der Burg, Sjoerd H.; Grabscheid, Benno; Hunziker, Isabelle P.; Kwappenberg, Kitty M.C.; Reichen, Jürg; Melief, Cornelis J.M.; Cerny, Andreas
Title: Molecular Mimicry of Human Cytochrome P450 by Hepatitis C Virus at the Level of Cytotoxic T Cell Recognition Document date: 1999_7_19
ID: 1vabzhs5_10
Snippet: Peptide "Stripping" by Mild Acid Treatment. JY cells were washed twice with PBS and then put on ice for 5 min. 10 7 cells were then treated for 90 s with 2 ml ice-cold citric acid-Na 2 HPO 4 buffer (a mixture of an equal volume of 0.263 M citric acid and 0.123 M Na 2 HPO 4 ), pH 3.2. Immediately thereafter, the eluted cells were buffered with cold IMDM, washed with IMDM, and resuspended at 5 ϫ 10 5 cells in IMDM with 1 g/ml ⤠2 -microglobulin .....
Document: Peptide "Stripping" by Mild Acid Treatment. JY cells were washed twice with PBS and then put on ice for 5 min. 10 7 cells were then treated for 90 s with 2 ml ice-cold citric acid-Na 2 HPO 4 buffer (a mixture of an equal volume of 0.263 M citric acid and 0.123 M Na 2 HPO 4 ), pH 3.2. Immediately thereafter, the eluted cells were buffered with cold IMDM, washed with IMDM, and resuspended at 5 ϫ 10 5 cells in IMDM with 1 g/ml ⤠2 -microglobulin (Sigma Chemical Co.).
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