Author: Kim, Sae-Hae; Cho, Byeol-Hee; Lee, Kyung-Yeol; Jang, Yong-Suk
Title: N-terminal Domain of the Spike Protein of Porcine Epidemic Diarrhea Virus as a New Candidate Molecule for a Mucosal Vaccine Document date: 2018_6_15
ID: 4vx7ez7s_10
Snippet: Whole-mount PPs prepared from the small intestines of BALB/c mice were incubated with recombinant NTD 231-501 protein (1 μg) for 2 h, fixed with 4% paraformaldehyde for 48 h, blocked with 3% bovine serum albumin (BSA) and 0.1% glycine in PBS, and then stained with anti-M cell Ab (NKM 16-2-4) followed by Alexa 488-conjugated anti-rat Ab, anti-GST Ab followed by Alexa 594-conjugated anti-rabbit Ab, or Alexa 405-conjugated wheat-germ agglutinin (WG.....
Document: Whole-mount PPs prepared from the small intestines of BALB/c mice were incubated with recombinant NTD 231-501 protein (1 μg) for 2 h, fixed with 4% paraformaldehyde for 48 h, blocked with 3% bovine serum albumin (BSA) and 0.1% glycine in PBS, and then stained with anti-M cell Ab (NKM 16-2-4) followed by Alexa 488-conjugated anti-rat Ab, anti-GST Ab followed by Alexa 594-conjugated anti-rabbit Ab, or Alexa 405-conjugated wheat-germ agglutinin (WGA). Specimens were analyzed by confocal laser scanning microscopy (LSM 510 META; Carl Zeiss, Oberkochen, Germany).
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