Author: Li, Yuetao; Zhao, Yongkun; Wang, Cuiling; Zheng, Xuexing; Wang, Hualei; Gai, Weiwei; Jin, Hongli; Yan, Feihu; Qiu, Boning; Gao, Yuwei; Li, Nan; Yang, Songtao; Xia, Xianzhu
Title: Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method Document date: 2018_3_23
ID: 4nphwznx_32
Snippet: RVFV pseudoviruses were reacted with serial dilutions of positive mouse serum to measure the neutralizing antibody titer. As shown in Table 1 , positive serum samples from mice immunized with RVFV proteins all had 100% neutralization activity in the 2-to 16-fold dilution range (indicating inhibition of RVFV pseudovirus infectious activity). Serum that was diluted 32-fold neutralized 70% of the virus infection, while 64-fold serum dilution neutral.....
Document: RVFV pseudoviruses were reacted with serial dilutions of positive mouse serum to measure the neutralizing antibody titer. As shown in Table 1 , positive serum samples from mice immunized with RVFV proteins all had 100% neutralization activity in the 2-to 16-fold dilution range (indicating inhibition of RVFV pseudovirus infectious activity). Serum that was diluted 32-fold neutralized 70% of the virus infection, while 64-fold serum dilution neutralized approximately 45% of the virus infection, and 128-fold serum dilution neutralized approximately 15% of the virus infection. However, the RVFV-positive mouse serum did not inhibit VSV pseudovirus infection. These results indicated that the RVFV pseudovirus neutralization assay could be used to effectively measure antibody neutralization; thus, this assay could be used to evaluate the anti-infection immune status in humans.
Search related documents:
Co phrase search for related documents- antibody neutralization and infectious activity: 1
- antibody neutralization and mouse serum: 1, 2, 3, 4, 5
- antibody neutralization and neutralization activity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45
- antibody neutralization and positive mouse serum: 1
- antibody neutralization and pseudovirus infection: 1, 2, 3, 4, 5
- antibody neutralization and pseudovirus neutralization: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
- antibody neutralization and RVFV protein: 1, 2
- antibody neutralization and RVFV pseudovirus neutralization: 1, 2
- antibody neutralization and RVFV pseudovirus neutralization assay: 1, 2
- antibody neutralization and serum dilution: 1, 2, 3, 4, 5, 6, 7, 8, 9
- antibody neutralization and serum sample: 1, 2
- antibody neutralization and virus infection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61
- antibody neutralization measure and pseudovirus neutralization: 1
- antibody titer and immune status: 1, 2, 3, 4, 5
- antibody titer and infectious activity: 1
- antibody titer and mouse serum: 1, 2
- antibody titer and neutralization activity: 1, 2, 3, 4, 5, 6
- antibody titer and pseudovirus neutralization: 1, 2, 3, 4
- antibody titer and serial dilution: 1, 2
Co phrase search for related documents, hyperlinks ordered by date