Selected article for: "expression plasmid and luciferase reporter plasmid"
Author: Lew, Qiao Jing; Chu, Kai Ling; Lee, Jialing; Koh, Poh Ling; Rajasegaran, Vikneswari; Teo, Jin Yuan; Chao, Sheng-Hao
Title: PCAF interacts with XBP-1S and mediates XBP-1S-dependent transcription
  • Document date: 2010_9_4
    • ID: 174q2pjw_7
    Snippet: Transient transfections of DNA plasmids into HEK293, 293T and MCF7 cells were performed using FuGENE 6 (Roche) according to the manufacturers' instructions. To perform the cell-based overexpression assays, cells were grown to 50-80% confluence in 96-well plates and co-transfected with a luciferase reporter and an expression plasmid. Lipofectamine 2000 Reagent (Invitrogen) was utilized to co-transfect cells with DNA plasmids and siRNAs for the cel.....
    Document: Transient transfections of DNA plasmids into HEK293, 293T and MCF7 cells were performed using FuGENE 6 (Roche) according to the manufacturers' instructions. To perform the cell-based overexpression assays, cells were grown to 50-80% confluence in 96-well plates and co-transfected with a luciferase reporter and an expression plasmid. Lipofectamine 2000 Reagent (Invitrogen) was utilized to co-transfect cells with DNA plasmids and siRNAs for the cell-based knockdown experiments. Firefly luciferase activities were measured 48 h post-transfection using the Bright-Glo assay system (Promega) and the activities were determined using an Infinite 200 multiplate reader (Tecan). HEK293 cells were used in the cell-based luciferase assays.

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