Selected article for: "positive result and sequence analysis"
Author: Cordey, Samuel; Thomas, Yves; Suter, Patricia; Kaiser, Laurent
Title: Pilot Evaluation of RT-PCR/Electrospray Ionization Mass Spectrometry (PLEX-ID/Flu assay) on Influenza-Positive Specimens
  • Document date: 2012_5_9
    • ID: 085v7n6k_8
    Snippet: Based on a selection of positive NPS collected in Switzerland during the 2010-2011 influenza season, this study suggests that the PLEX-ID/Flu assay is a convenient platform for the detection, typing, and subtyping (lineage characterization for influenza B) of circulating influenza viruses. The detection rate observed with the PLEX-ID platform, as well as the subtyping or lineage characterization of human influenza virus types A and B tends to sug.....
    Document: Based on a selection of positive NPS collected in Switzerland during the 2010-2011 influenza season, this study suggests that the PLEX-ID/Flu assay is a convenient platform for the detection, typing, and subtyping (lineage characterization for influenza B) of circulating influenza viruses. The detection rate observed with the PLEX-ID platform, as well as the subtyping or lineage characterization of human influenza virus types A and B tends to suggest that it might play a role in the near future in most routine laboratories, but this needs to be confirmed in prospective and large comparative studies. In addition, the sensitivity of the PLEX-ID/Flu observed in this study could be increased if any positive PCR results were considered and if the typing result is not a requirement. However, this pilot study has intrinsic limitations mainly related to the fact that only realtime RT-PCR-positive specimens were selected initially. This precludes any appropriate evaluation of the respective sensitivity or specificity of the method. Compared to sequence analysis, the PLEX-ID/Flu assay has also limitations in terms of identification of specific mutations potentially involved in antiviral resistance, as well as drifted strains. Finally, the lack of accurate typing concerning the HA and NA genes is a major drawback and possibly limits the use of this assay as a first line diagnostic tests.

    Search related documents:
    Co phrase search for related documents
    • positive PCR result and routine laboratory: 1, 2
    • positive PCR result and RT PCR positive specimen: 1, 2, 3, 4
    • positive PCR result and sequence analysis: 1, 2
    • respective sensitivity and specificity respective sensitivity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • role play and routine laboratory: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • role play and sequence analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47
    • role play and specific mutation: 1, 2, 3, 4, 5, 6, 7
    • role play and study observe: 1
    • role play and study suggest: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29
    • routine laboratory and sequence analysis: 1, 2
    • routine laboratory and specific mutation: 1, 2
    • routine laboratory and study suggest: 1
    • sequence analysis and specific mutation: 1, 2
    • sequence analysis and study observe: 1
    • sequence analysis and study suggest: 1, 2, 3, 4, 5