Preclinical safety and tolerability of a repeatedly administered human leishmaniasis DNA vaccine

Riede, O; Seifert, K; Oswald, D; Endmann, A; Hock, C; Winkler, A; Salguero, F J; Schroff, M; Croft, S L; Juhls, C

MIDGE-Th1 vectors persist at injection site and in draining lymph nodes Persistence of MIDGE-Th1 vectors was examined 14 days and 60 days post treatment. Fourteen days after four injections LEISHDNAVAX was cleared from the majority of organs (Figure 1c ). High vector copy numbers per μg total DNA were found in skin of injection site (all animals positive, geometric mean: 3.45 × 10 6 copies) and in inguinal lymph nodes (7/10 animals positive, geometric mean: 2.88 × 10 4 copies). Sixty days after four injections, MIDGE-Th1 vectors were cleared from most tissues but persisted in inguinal lymph nodes (5/10 animals positive, geometric mean: 1.44 × 10 4 copies) and skin of injection site (all animals positive, geometric mean: 6.93 × 10 5 copies) (Figure 1d ).