Title: Primary sequence domains required for the retention of rotavirus VP7 in the endoplasmic reticulum Document date: 1988_11_1
ID: 63mxzwti_7
Snippet: The plasmid pMSal04 (12) containing the full length cDNA coding sequence of mouse salivary a-amylase (a gift from O. Hagenbuchle, Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland), was used in the construction of both the amylase transcription vector Am/ pGEM3 (pGEM3 was obtained from Promega Biotec, Madison, WI), and the expression vector pJC/Am. The plasmid pMSal04 was cut with Pst I and the 1,626-bp fragment containing t.....
Document: The plasmid pMSal04 (12) containing the full length cDNA coding sequence of mouse salivary a-amylase (a gift from O. Hagenbuchle, Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland), was used in the construction of both the amylase transcription vector Am/ pGEM3 (pGEM3 was obtained from Promega Biotec, Madison, WI), and the expression vector pJC/Am. The plasmid pMSal04 was cut with Pst I and the 1,626-bp fragment containing the full length amylase coding region was isolated and cloned into the Pst I site of the pGEM3 transcription vector.
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