Propagation of Human Embryonic Stem Cells on Human Amniotic Fluid Cells as Feeder Cells in Xeno-Free Culture Conditions

Jung, Juwon; Baek, Jin Ah; Seol, Hye Won; Choi, Young Min

In the present study, we aimed to determine whether (Stacey et al., 2006) . Several factors should be tested for in human derived materials or cells to be used as biological medicines (Cobo et al., 2005) Recently, studies in hPSCs, including hESCs, have focused on the next stage in clinical application, necessitating compatibility of cell therapy products with clinical application, the final goal of stem cell studies. Therefore, in addition to these changes, efficient culture conditions must be established for growth of hESCs by reducing animal derived components and replacing them with humanized or human derived materials in the basic cell culture environment. Studies should also be conducted with xeno-free and feeder-free environments that culture without feeder cells. In addition to improvement of culture conditions, quality management of cultured hESCs and standard procedures, including detection of microorganisms, viruses, and animal derived materials, for identification of inappropriate harmful substances need to be developed and standardized for production of clinical grade hESCs. This is the next preparatory step for the application of hESCs as cell based therapy in regenerative medicine.