Author: Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.
Title: Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus Document date: 2010_7_20
ID: 2zhaknbi_26
Snippet: For quantitative analysis, previous investigations using SILAC and LC-MS/MS have applied ratio cutoffs ranging from near 1.3-to 2.0-fold (41) . A previous study that investigated changes in the nuclear proteins in HRSV-infected cells used a ratio cutoff of 2.0-fold for comparison (26) . In this study, a 2.0-fold cutoff was chosen as a basis for investigating potential proteome changes between data sets through IPA and to provide a basis for compa.....
Document: For quantitative analysis, previous investigations using SILAC and LC-MS/MS have applied ratio cutoffs ranging from near 1.3-to 2.0-fold (41) . A previous study that investigated changes in the nuclear proteins in HRSV-infected cells used a ratio cutoff of 2.0-fold for comparison (26) . In this study, a 2.0-fold cutoff was chosen as a basis for investigating potential proteome changes between data sets through IPA and to provide a basis for comparing the current data set with previous studies. The comparison of mock-infected with HRSV-infected cells indicated that in the nuclear fraction many proteins showed a 2-fold or greater decrease in abundance (supplemental Table 1 ), whereas in the cytoplasmic fraction, very few proteins changed in abundance (supplemental Table 2 ).
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