Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus

Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.

The quantitative proteomic analysis indicated that in HRSVinfected cells nucleoporins (nups) were depleted; e.g. nup96 and nup98 were depleted 3.6-fold (and those shown in Fig. 10A ). The only exceptions were nup85 and nup160. Indirect immunofluorescence confocal microscopy was used to investigate the subcellular localization of the nuclear protein lamin B in mock-infected cells in comparison with HRSV-infected cells (Fig. 10B) . The data indicated that in mock-infected cells the protein was localized to the nuclear envelope but also was distributed between the nucleus and the cytoplasm. In contrast, in HRSV-infected cells, lamin B appeared to be more concentrated around the nuclear envelope with less fluorescence observed in the cytoplasm or the nucleus, which is indicative of altered trafficking or loss of nuclear pore complex function.