Author: Hollien, Julie; Lin, Jonathan H.; Li, Han; Stevens, Nicole; Walter, Peter; Weissman, Jonathan S.
Title: Regulated Ire1-dependent decay of messenger RNAs in mammalian cells Document date: 2009_8_10
ID: 3gwm1c2f_18
Snippet: Establishment of Ire1-expressing cell lines and RNAi Ire1-î¡ î€²/ MEFs were obtained from R. Kaufman (University of Michigan Medical Center, Ann Arbor, MI), and XBP1 / MEFs were obtained from L. Glimcher (Harvard School of Public Health, Boston, MA). All cells were maintained at 37°C and 5% CO 2 in DME supplemented with 10% fetal calf serum, Gln, and antibiotics. We generated Ire1-î¡ î€²/ MEFs containing induction of ERdj4 by DTT, .....
Document: Establishment of Ire1-expressing cell lines and RNAi Ire1-î¡ î€²/ MEFs were obtained from R. Kaufman (University of Michigan Medical Center, Ann Arbor, MI), and XBP1 / MEFs were obtained from L. Glimcher (Harvard School of Public Health, Boston, MA). All cells were maintained at 37°C and 5% CO 2 in DME supplemented with 10% fetal calf serum, Gln, and antibiotics. We generated Ire1-î¡ î€²/ MEFs containing induction of ERdj4 by DTT, RIDD remained intact when cells were treated with both 1NM-PP1 and DTT (Fig. 4 F) .
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