Author: Hollien, Julie; Lin, Jonathan H.; Li, Han; Stevens, Nicole; Walter, Peter; Weissman, Jonathan S.
Title: Regulated Ire1-dependent decay of messenger RNAs in mammalian cells Document date: 2009_8_10
ID: 3gwm1c2f_25
Snippet: To deplete cells of Ire1 or XBP-1 by RNAi, we transfected îº3 × 10 4 cells with 150 ng total of a mixture of four different siRNAs (QIAGEN). After 48 h, we treated cells with DTT and/or 1NM-PP1, and purified RNA as described in the next section. We typically attained 90% knockdown as measured by qPCR......
Document: To deplete cells of Ire1 or XBP-1 by RNAi, we transfected îº3 × 10 4 cells with 150 ng total of a mixture of four different siRNAs (QIAGEN). After 48 h, we treated cells with DTT and/or 1NM-PP1, and purified RNA as described in the next section. We typically attained 90% knockdown as measured by qPCR.
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