Selected article for: "chain reaction and DNA polymerase"
Author: Tromas, Nicolas; Zwart, Mark P.; Forment, Javier; Elena, Santiago F.
Title: Shrinkage of Genome Size in a Plant RNA Virus upon Transfer of an Essential Viral Gene into the Host Genome
  • Document date: 2014_2_20
    • ID: 5fejitls_7
    Snippet: The pTEV7DA infectious clone (Dolja et al. 1992 ) was used as a source for TEV. A TEV genotype was produced that lacked the full replicase gene (ÁNIb) by inverse polymerase chain reaction (PCR) using Pfu turbo DNA polymerase (Stratagene) and primers conserving the proteolytic NIa/NIb (ÁNIb-F) and NIb/CP (ÁNIb-R) sites (supplementary table S1, Supplementary Material online). The resulting clone was named pTEV7DA-DNIb (Tromas and Elena 2010) ......
    Document: The pTEV7DA infectious clone (Dolja et al. 1992 ) was used as a source for TEV. A TEV genotype was produced that lacked the full replicase gene (ÁNIb) by inverse polymerase chain reaction (PCR) using Pfu turbo DNA polymerase (Stratagene) and primers conserving the proteolytic NIa/NIb (ÁNIb-F) and NIb/CP (ÁNIb-R) sites (supplementary table S1, Supplementary Material online). The resulting clone was named pTEV7DA-DNIb (Tromas and Elena 2010) .

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