Selected article for: "protein level and western blot"
Author: Sousa, Carole; Golebiewska, Anna; Poovathingal, Suresh K; Kaoma, Tony; Pires-Afonso, Yolanda; Martina, Silvia; Coowar, Djalil; Azuaje, Francisco; Skupin, Alexander; Balling, Rudi; Biber, Knut; Niclou, Simone P; Michelucci, Alessandro
Title: Single-cell transcriptomics reveals distinct inflammation-induced microglia signatures
  • Document date: 2018_9_11
    • ID: 0662rivp_4
    Snippet: a. An untreated group should be included (i.e. non-saline or -LPS injected) b. Validation on RNA expression (e.g. qPCR, in situ hybridisation) or protein level (e.g. Western blot, histology) should be undertaken, especially of the genes mentioned in the discussion (SOCS3, STAT3, Mef2c, TREM2, TYROBP) c. The relevance of the identified genes should be discussed in more detail when comparing the three microglia subsets to each other and to the DAM-.....
    Document: a. An untreated group should be included (i.e. non-saline or -LPS injected) b. Validation on RNA expression (e.g. qPCR, in situ hybridisation) or protein level (e.g. Western blot, histology) should be undertaken, especially of the genes mentioned in the discussion (SOCS3, STAT3, Mef2c, TREM2, TYROBP) c. The relevance of the identified genes should be discussed in more detail when comparing the three microglia subsets to each other and to the DAM-specific signature. What are the functional implications? Is the LPS-response of microglia harmful or beneficial towards tissue homeostasis and repair? d. Do the microglia of the "LPS-subset" share common markers that can be used to selectively isolate and analyse this specific population? It would give the study strength if the authors could confirm the activated cells of "subset LPS" in the CNS in vivo using the up and downregulated genes.

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