Author: Sousa, Carole; Golebiewska, Anna; Poovathingal, Suresh K; Kaoma, Tony; Pires-Afonso, Yolanda; Martina, Silvia; Coowar, Djalil; Azuaje, Francisco; Skupin, Alexander; Balling, Rudi; Biber, Knut; Niclou, Simone P; Michelucci, Alessandro
Title: Single-cell transcriptomics reveals distinct inflammation-induced microglia signatures Document date: 2018_9_11
ID: 0662rivp_40
Snippet: To include the requested control, we used the Monocyte Cell Isolation Kit (Miltenyi Biotec) that has been developed for the isolation of monocytes from suspensions of mouse bone marrow. Remarkably, FACS analyses revealed pure populations of Ly6C + cells after depletion of magnetically labelled non-target cells, i.e. T cells, B cells, NK cells, dendritic cells, erythroid cells and granulocytes. We included the FACS results in Fig. EV2 and the corr.....
Document: To include the requested control, we used the Monocyte Cell Isolation Kit (Miltenyi Biotec) that has been developed for the isolation of monocytes from suspensions of mouse bone marrow. Remarkably, FACS analyses revealed pure populations of Ly6C + cells after depletion of magnetically labelled non-target cells, i.e. T cells, B cells, NK cells, dendritic cells, erythroid cells and granulocytes. We included the FACS results in Fig. EV2 and the corresponding qPCR data in Fig. 2D , which further support the validity of our FACS gating strategy and purity of FACS-sorted cells. We revised the text and the figure legends accordingly.
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