Author: Sousa, Carole; Golebiewska, Anna; Poovathingal, Suresh K; Kaoma, Tony; Pires-Afonso, Yolanda; Martina, Silvia; Coowar, Djalil; Azuaje, Francisco; Skupin, Alexander; Balling, Rudi; Biber, Knut; Niclou, Simone P; Michelucci, Alessandro
Title: Single-cell transcriptomics reveals distinct inflammation-induced microglia signatures Document date: 2018_9_11
ID: 0662rivp_70
Snippet: We acknowledge the reviewer for this advice, which is in line with Referee #1 comment (please refer to major point 3b). We took advantage of primary cells to analyse Socs3 at gene expression level by qPCR and to investigate STAT3 phosphorylation levels by Western blot. Microglial cells treated with LPS showed increased amounts of STAT3 phosphorylation along with up-regulation of Socs3 expression levels compared to untreated cells (Appendix Fig. S.....
Document: We acknowledge the reviewer for this advice, which is in line with Referee #1 comment (please refer to major point 3b). We took advantage of primary cells to analyse Socs3 at gene expression level by qPCR and to investigate STAT3 phosphorylation levels by Western blot. Microglial cells treated with LPS showed increased amounts of STAT3 phosphorylation along with up-regulation of Socs3 expression levels compared to untreated cells (Appendix Fig. S1 ).
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