Author: Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas; Hacker, Jill; Armstrong, R. Todd; White, Judith M.
Title: Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity Document date: 1998_6_15
ID: 78fjem8s_49
Snippet: were assayed in parallel for HA cell surface expression, cleavability with trypsin, and fusion activity. Fusion was monitored at both 2 (Fig. 7 C) and 10 ( Fig. 7 D) min of acidification. With decreasing amounts of wt-HA, fusion activity decreased; with increasing amounts of V55P, fusion activity increased. When normalized for cell surface levels of HA1, the fusion activity of V55P was ‫02Ù‬ and ‫%05Ù‬ that of wt-HA at 2 and 10 min, res.....
Document: were assayed in parallel for HA cell surface expression, cleavability with trypsin, and fusion activity. Fusion was monitored at both 2 (Fig. 7 C) and 10 ( Fig. 7 D) min of acidification. With decreasing amounts of wt-HA, fusion activity decreased; with increasing amounts of V55P, fusion activity increased. When normalized for cell surface levels of HA1, the fusion activity of V55P was ‫02Ù‬ and ‫%05Ù‬ that of wt-HA at 2 and 10 min, respectively. These latter observations suggested that the rate of fusion of V55P is slow compared with wt-HA.
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