Author: Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas; Hacker, Jill; Armstrong, R. Todd; White, Judith M.
Title: Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity Document date: 1998_6_15
ID: 78fjem8s_60
Snippet: Within our mutant set, we analyzed four HAs with either single (V55P, S71P, L80P) or double (V55P/S71P) Pro substitutions in the region of high coiled-coil propensity. Given that residue 71 falls in a "b" position of the final coiled-coil, we reasoned that it would have, at most, a modest effect on fusion. Given that residues 55 and 80 fall in "d" positions, we predicted that both mutations would impair fusion. We further predicted that the doubl.....
Document: Within our mutant set, we analyzed four HAs with either single (V55P, S71P, L80P) or double (V55P/S71P) Pro substitutions in the region of high coiled-coil propensity. Given that residue 71 falls in a "b" position of the final coiled-coil, we reasoned that it would have, at most, a modest effect on fusion. Given that residues 55 and 80 fall in "d" positions, we predicted that both mutations would impair fusion. We further predicted that the double mutant (V55P/S71P) would be severely impaired for fusion. We found the following: S71P behaved like wt-HA. L80P mediated lipid and content mixing like wt-HA but appeared to be somewhat impaired in syncytia formation.
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