Author: Deng, Zengqin; Lehmann, Kathleen C.; Li, Xiaorong; Feng, Chong; Wang, Guoqiang; Zhang, Qi; Qi, Xiaoxuan; Yu, Lin; Zhang, Xingliang; Feng, Wenhai; Wu, Wei; Gong, Peng; Tao, Ye; Posthuma, Clara C.; Snijder, Eric J.; Gorbalenya, Alexander E.; Chen, Zhongzhou
Title: Structural basis for the regulatory function of a complex zinc-binding domain in a replicative arterivirus helicase resembling a nonsense-mediated mRNA decay helicase Document date: 2013_12_24
ID: 471zei5o_13
Snippet: The structure of nsp10Ã was determined by the MAD method. Initial phases were calculated by SOLVE, and phases were subsequently improved using RESOLVE (42) . The figure of merit from the MAD phasing was 0.36 and the Z score was 15.7. Several segments of the protein could be automatically modelled into the electron-density map by RESOLVE, although in part only as poly-alanine chains. Manual rebuilding was performed in COOT (43) , and refinement w.....
Document: The structure of nsp10Ã was determined by the MAD method. Initial phases were calculated by SOLVE, and phases were subsequently improved using RESOLVE (42) . The figure of merit from the MAD phasing was 0.36 and the Z score was 15.7. Several segments of the protein could be automatically modelled into the electron-density map by RESOLVE, although in part only as poly-alanine chains. Manual rebuilding was performed in COOT (43) , and refinement was performed with REFAMC5 (44) . Further rounds of refinement were done with Translation/Libration/Screw (TLS) refinement (45) . The structure was refined to 2.0 Ã… with an R work of 19.5% and an R free of 22.4%.
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