Selected article for: "dna polymerase and PCR amplification"
Author: Sztuba-Solinska, Joanna; Teramoto, Tadahisa; Rausch, Jason W.; Shapiro, Bruce A.; Padmanabhan, Radhakrishnan; Le Grice, Stuart F. J.
Title: Structural complexity of Dengue virus untranslated regions: cis-acting RNA motifs and pseudoknot interactions modulating functionality of the viral genome
  • Document date: 2013_3_26
    • ID: 1pbd4maf_7
    Snippet: In vitro RNA synthesis DNA templates for in vitro transcription were generated by PCR amplification of the plasmid DENV-MINI and the corresponding mutants, using primers listed in Supplementary Table S1 . All PCR experiments were performed using Invitrogen Platinum Õ Taq DNA polymerase High Fidelity. Transcripts were synthesized with the Ambion T7-MEGAscript following the manufacturer's protocol, and RNAs were purified from denaturating 8 M urea.....
    Document: In vitro RNA synthesis DNA templates for in vitro transcription were generated by PCR amplification of the plasmid DENV-MINI and the corresponding mutants, using primers listed in Supplementary Table S1 . All PCR experiments were performed using Invitrogen Platinum Õ Taq DNA polymerase High Fidelity. Transcripts were synthesized with the Ambion T7-MEGAscript following the manufacturer's protocol, and RNAs were purified from denaturating 8 M urea/5% polyacrylamide gels, followed by elution and ethanol precipitation. Purified RNAs were dissolved in sterile water and stored at À20 C.

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