Title: Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway Document date: 1995_10_1
ID: 7oklz2ch_50
Snippet: First, we identified the signals involved in pre-medial-Golgi localization by a biochemical approach under high expression conditions, and second, we tested whether the signals defined by this assay were required for the correct localization of ERGIC-53 within the ER-ERGIC-cis-Golgi system. For this purpose we analyzed by immunofluorescence microscopy the distribution of mutant ERGIC-53 proteins expressed at moderate expression levels, which is t.....
Document: First, we identified the signals involved in pre-medial-Golgi localization by a biochemical approach under high expression conditions, and second, we tested whether the signals defined by this assay were required for the correct localization of ERGIC-53 within the ER-ERGIC-cis-Golgi system. For this purpose we analyzed by immunofluorescence microscopy the distribution of mutant ERGIC-53 proteins expressed at moderate expression levels, which is three times on average the level of endogenous ERGIC-53. This second analysis confirmed that the signals identifled under high expression conditions are true targeting determinants and not simply an artefactual result due to excessive overexpression.
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