Title: Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6 Document date: 1992_11_1
ID: 3ymo8zw0_7
Snippet: Purification of Human NK Cells. Highly enriched populations of NK cells from peripheral blood were purified by discontinuous Percoll gradient centrifugation (Pharmacia Fine Chemicals, Uppsala, Sweden) as previously described (17) . In several experiments, contaminating T cells were eliminated by panning with anti-CD5 mAb, or by rigorous immunomagnetic negative selection with a cocktail of mAb against CD3 and CD14 antigens . The resulting NK cell .....
Document: Purification of Human NK Cells. Highly enriched populations of NK cells from peripheral blood were purified by discontinuous Percoll gradient centrifugation (Pharmacia Fine Chemicals, Uppsala, Sweden) as previously described (17) . In several experiments, contaminating T cells were eliminated by panning with anti-CD5 mAb, or by rigorous immunomagnetic negative selection with a cocktail of mAb against CD3 and CD14 antigens . The resulting NK cell populations were more than 90% CD16', CD56', CD3 -, and CD14 -, as assessed by cytofluorimetric analysis .
Search related documents:
Co phrase search for related documents- cell population and highly enriched population: 1, 2, 3, 4
- cell population and negative selection: 1
- cell population and NK cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- cell population and NK cell population: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- cell population and peripheral blood: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- cytofluorimetric analysis and NK cell: 1
- gradient centrifugation and highly enriched population: 1
- gradient centrifugation and peripheral blood: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- mAb cocktail and NK cell: 1
- mAb cocktail and peripheral blood: 1
- negative selection and NK cell: 1
- negative selection and peripheral blood: 1, 2, 3, 4, 5, 6, 7
- NK cell and peripheral blood: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61
- NK cell population and peripheral blood: 1
Co phrase search for related documents, hyperlinks ordered by date