Author: Hölzer, Martin; Schoen, Andreas; Wulle, Julia; Müller, Marcel A.; Drosten, Christian; Marz, Manja; Weber, Friedemann
Title: Virus- and Interferon Alpha-Induced Transcriptomes of Cells from the Microbat Myotis daubentonii Document date: 2019_8_10
ID: 0co6m9af_63
Snippet: Principal component analyses were performed using the PCAGO web service (https://doi.org/10.1101/433078). As input, unique read counts of all 18 samples were used and DESeq2-normalized and rlog-transformed with the build in functionalities of the web service. Genes with zero or constant read counts were removed prior PCA and the top 500 variant genes were finally used for the transformation shown in Fig.3 . Supplemental 2d-and 3d-PCA movies were .....
Document: Principal component analyses were performed using the PCAGO web service (https://doi.org/10.1101/433078). As input, unique read counts of all 18 samples were used and DESeq2-normalized and rlog-transformed with the build in functionalities of the web service. Genes with zero or constant read counts were removed prior PCA and the top 500 variant genes were finally used for the transformation shown in Fig.3 . Supplemental 2d-and 3d-PCA movies were generated starting with the top 10 variant genes and adding step-wise 10 genes until reaching all 21,791 genes without zero or constant read count.
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