Selected article for: "pcr assay and real time"
Title: 10th Annual European College of Equine Internal Medicine Congress: 2—4 November, 2017
  • Document date: 2018_2_22
    • ID: zk6ttiq1_31
    Snippet: Methods: In a prospective study two adult female donkeys and one control horse were inoculated intravenously with purified EqHV from a naturally infected horse. Animals underwent daily clinical examinations and blood was drawn at weekly intervals for 15 weeks. Haematological data, GLDH, GGT, AST and fibrinogen were evaluated. In serum anti-NS3-EqHV antibodies were analysed by luciferase immunoprecipitation assay and viral load was quantified usin.....
    Document: Methods: In a prospective study two adult female donkeys and one control horse were inoculated intravenously with purified EqHV from a naturally infected horse. Animals underwent daily clinical examinations and blood was drawn at weekly intervals for 15 weeks. Haematological data, GLDH, GGT, AST and fibrinogen were evaluated. In serum anti-NS3-EqHV antibodies were analysed by luciferase immunoprecipitation assay and viral load was quantified using real-time PCR. Liver biopsies were taken before inoculation and after EqHV PCR positivity in serum.

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