Title: 10th Annual European College of Equine Internal Medicine Congress: 2—4 November, 2017 Document date: 2018_2_22
ID: zk6ttiq1_40
Snippet: Objectives: To examine the diagnostic value of a novel qPCR assay designed to detect equine dermatophyte infections Methods: A qPCR assay was designed to detect targets within chitin sythetase and beta-tubulin genes considered to be specific for dermatophyte species including those known to affect equids. The assay was used on stored frozen hair/skin samples which had previously been subject to culture using Saboraud's agar (SAB) and also prospec.....
Document: Objectives: To examine the diagnostic value of a novel qPCR assay designed to detect equine dermatophyte infections Methods: A qPCR assay was designed to detect targets within chitin sythetase and beta-tubulin genes considered to be specific for dermatophyte species including those known to affect equids. The assay was used on stored frozen hair/skin samples which had previously been subject to culture using Saboraud's agar (SAB) and also prospectively in freshly submitted samples of hair/skin that were also subject to culture on SAB. Six different DNA extraction methods were used over time.
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