Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_126
Snippet: Three glioma cell lines (J3TBg, G06A, SDT3G) were used to validate targeting of peptides to live cells using flow cytometry and immunofluorescent labelled peptide. Canine glioma cell derived exosomes were isolated from serum free media using ExoQuick precipitation and centrifugation, and characterized using nanoparticle tracking analysis (NTA) technology (NanoSight Ã’ LM10HS) and peptide conjugated quantum dots. Non-glioma targeting peptide (LLP2.....
Document: Three glioma cell lines (J3TBg, G06A, SDT3G) were used to validate targeting of peptides to live cells using flow cytometry and immunofluorescent labelled peptide. Canine glioma cell derived exosomes were isolated from serum free media using ExoQuick precipitation and centrifugation, and characterized using nanoparticle tracking analysis (NTA) technology (NanoSight Ã’ LM10HS) and peptide conjugated quantum dots. Non-glioma targeting peptide (LLP2A/a4b1) was used as a negative control.
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