Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_198
Snippet: A biorepository maintained by the Purdue Comparative Oncology Program was searched for RNA samples of histopathologically-confirmed cases of canine DLBCL diagnosed between 2010 and 2015. Archived lymph node biopsy samples, processed in TRIzol reagent, from treatment-na€ ıve dogs were retrieved. Paired RNA samples from lymph node from clinically healthy dogs were retrieved to serve as controls. Multiplex qPCR was performed using TaqMan probes a.....
Document: A biorepository maintained by the Purdue Comparative Oncology Program was searched for RNA samples of histopathologically-confirmed cases of canine DLBCL diagnosed between 2010 and 2015. Archived lymph node biopsy samples, processed in TRIzol reagent, from treatment-na€ ıve dogs were retrieved. Paired RNA samples from lymph node from clinically healthy dogs were retrieved to serve as controls. Multiplex qPCR was performed using TaqMan probes and primers against canine PD-L1 and GAPDH. The data were analyzed using the 2 -DDCT method to calculate the fold change in PD-L1 expression in DLBCL relative to normal lymph node.
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