2017 ACVIM Forum Research Abstract Program

Serum SDMA concentrations did not distinguish hyperthyroid cats with and without azotemic CKD. The influence of hyperthyroidism on serum SDMA concentrations warrants further investigation. Obesity is a frequent nutritional disorder in dogs and it is associated with several metabolic alterations and represents a risk factor for several systemic comorbidities. However, there is controversy about which of the adipose tissues contributes more to these complications in the canine species. The present study aimed to evaluate the subcutaneous and visceral adipose tissue of ten obese female dogs and ten female dogs with eutrophic body condition, regarding adipocyte size and presence of leukocyte infiltration. The study was conducted with routine animals from the Veterinary Hospital, and all of the animals were submitted to a complete clinical examination, classification based on the body condition score and blood exams to exclude concomitant diseases. Fragments of subcutaneous and visceral adipose tissue were obtained during electives ovariosalpingohisterectomy procedures, the samples were stained with Hematoxylin-Eosin and analyzed with Zeiss Microscope software. It was observed that obese dogs presented remarkably larger adipocyte size when compared to animals with ideal body condition, in a significance level of 5% through Student's T-test. Likewise, it was noted the presence of significantly higher leukocyte infiltrate in obese animals, in a significance level of 5% in the Kruskal-Wallis test, compared to animals with normal body condition that did not have leukocyte infiltration detected. However, the size of adipocytes and leukocyte infiltrate in obese animals did not differ significantly between the visceral and subcutaneous adipose tissue samples. It was concluded, therefore, that obesity in dogs is related with the increase of adipocytes and with presence of leukocyte infiltration, but it was not possible to state in this study that different fat deposits have differentiated metabolic and endocrine levels, which could interfere in a specific way in the inherent processes in obese and diabetic patients. Obesity is a frequent nutritional disorder in dogs and cats and it results in oxidative stress. The evaluation of oxidative stress can be performed through the process of lipid peroxidation, and malonate is one of the most abundant aldehydes. The evaluation method is called "test for substances that react with thiobarbituric acid" (TBARS). Objective of the present study is to evaluate oxidative stress by measuring the TBARS present in samples of obese dogs before and after the weight loss program. The work was carried out with routine animals of the Veterinary Hospital of the Pontifical Catholic University of Parana (located in Parana, Brazil). The animals were submitted to a complete clinical examination, blood count, biochemistry, urinalysis and systolic blood pressure measurement. Subsequently, they were classified as obese on the body scale; body mass index and percentage of body fat. The animals were divided into group 1 (pre-diet) and group 2 (post-diet). Ten obese animals were selected and submitted to a hypocaloric diet loss program. TBARS was performed using the Elisa technique. All animals in groups 1 and 2 presented TBARS values above the reference value, and there was a statistically significant reduction of the values from group 1 to group 2 (P = 0.034). Based on the results, it was concluded that obese dogs present higher p