2017 ACVIM Forum Research Abstract Program

Thirty ACTH stimulation tests were performed on 15 dogs divided into 2 groups: 5 control animals (2 females and 3 males, healthy, mean age 2.5 AE 1.5 years) and 10 treatment animals (6 females and 4 males, previously hyperadrenocorticism diagnosed, mean age 11.7 AE 2.3 years). All animals were controlling them, in other words, first were challenged with Synacthen and then with Acthelea. For dogs on trilostano (1-2 mg/kg/day), ACTH stimulation tests were initiated 4-6 hours postpill administration as per manufacturer instructions. The first stimulation tests were performed using Synacthen as the drug of election to induce cortisol response and the second, which were separated by 15 days period, using Acthelea. Blood samples were obtained just before injection and 60 minute post-ACTH. The first to assess the cortisol basal concentration of control group (CSynbas and CEleabas) and Treated group (TtoSynbas and TtoEleapos) and the second collection for assessment of cortisol concentration post-ACTH in the control group (CSynpos and CEleapos) and treated group (TtoSynpos and TtoEleapos).The doses were: 0.04 mL/Kg for Synacthen and 0.08 mL/Kg for Acthelea, IV. Serum cortisol concentrations were measured at the PROVET/BR Hormone Laboratory using previously validated radioimmunoassay (MP Biomedicals).