Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_316
Snippet: Fresh cadaveric samples were obtained from duodenum, jejunum and colon of seven individual dogs, followed by crypt isolation. Organoids were propagated in 3D-culture using RPSO1based expansion media. Differentiation was achieved by removing proliferation inducing components. We established a crypt isolation protocol and kept organoid lines from three areas of the intestine (duodenum, jejunum and colon) from seven dogs in culture during at least 1.....
Document: Fresh cadaveric samples were obtained from duodenum, jejunum and colon of seven individual dogs, followed by crypt isolation. Organoids were propagated in 3D-culture using RPSO1based expansion media. Differentiation was achieved by removing proliferation inducing components. We established a crypt isolation protocol and kept organoid lines from three areas of the intestine (duodenum, jejunum and colon) from seven dogs in culture during at least 12 passages. The gene-expression levels of stem cell markers (LGR5, CD133, ASCL2 and OLFM4) were stable in expansion media. After differentiation, expression of the goblet cell marker MUC2 and Paneth cell marker NEUROG3 were increased, whereas expression levels of stem cell marker LGR5 was decreased.
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