2017 ACVIM Forum Research Abstract Program

Intra-and inter-assay variability (median %CV) were 4% and 6% for cholesterol, 5% and 11% for coprostanol, 4% and 9% for cholestanol, 4% and 6% for b-sitosterol, 4% and 7% for stigmasterol, 4% and 6% for campesterol, 4% and 11% for sitostanol, 6% and 10% for fucosterol, 4% and 7% for palmitic acid, 4% and 9% for oleic acid, 8% and 10% for linoleic acid, 14% and 12% for a-linolenic acid, 4% and 6% for stearic acid, 5% and 11% for gondoic acid, and 10% and 18% for erucic acid. Limits of quantitation for each compound in lg/mL (LLOQ-ULOQ) were as follows: palmitic acid (6.25-800), linoleic acid (6.25-800), a-linolenic acid (12.5-400), oleic acid (12.5-800), stearic acid In conclusion, the GC/MS method validated here is precise, reproducible, and sensitive for the quantification of several sterols and fatty acids in canine fecal samples. Previous studies have identified dysbiosis in dogs with idiopathic IBD (IBD) and dogs with acute hemorrhagic diarrhea syndrome (AHDS). The aim of this study was to evaluate whether dysbiosis patterns differ between these disease groups, and over time.