Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_180
Snippet: MiR-34a expression was evaluated in primary canine OSA tumors, OSA cell lines and normal canine osteoblasts (Cell Applications, Inc.) using Taqman miRNA assays. To investigate the effects of miR-34a on OSA cell behavior, canine OSA8 and Abrams cell lines were stably transduced with empty control or pre-miR-34a lentiviral constructs (Systems Biosciences) and cell proliferation, cell cycling, cell invasion and migration was evaluated. RNA sequencin.....
Document: MiR-34a expression was evaluated in primary canine OSA tumors, OSA cell lines and normal canine osteoblasts (Cell Applications, Inc.) using Taqman miRNA assays. To investigate the effects of miR-34a on OSA cell behavior, canine OSA8 and Abrams cell lines were stably transduced with empty control or pre-miR-34a lentiviral constructs (Systems Biosciences) and cell proliferation, cell cycling, cell invasion and migration was evaluated. RNA sequencing of canine OSA8 cells overexpressing miR-34a was performed and changes in gene expression were validated using real-time PCR.
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