Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_358
Snippet: Live kittens (n = 61) with and without diarrhea and deceased kittens (n = 54) that were euthanized in apparent good health or were euthanized/died due to severe diarrhea were obtained from 2 different shelter facilities. All kittens were ≤ 12-wks of age and ≤1 kg body weight. Fecal culture for EPEC and extraction of fecal DNA for qPCR amplification of EPEC enterocyte-attaching-effacing gene (eae) was performed. Deceased kittens underwent auto.....
Document: Live kittens (n = 61) with and without diarrhea and deceased kittens (n = 54) that were euthanized in apparent good health or were euthanized/died due to severe diarrhea were obtained from 2 different shelter facilities. All kittens were ≤ 12-wks of age and ≤1 kg body weight. Fecal culture for EPEC and extraction of fecal DNA for qPCR amplification of EPEC enterocyte-attaching-effacing gene (eae) was performed. Deceased kittens underwent autopsy and sections of the stomach, small intestine, and colon were obtained for histopathology, scoring of lesions, and fluorescence in-situ hybridization (FISH) to detect enteroadherent E. coli. Medical records were examined for variables of potential association with EPEC infection.
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