Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_340
Snippet: Canine specific CD25 protein and antibodies were commercially available and used to establish this assay. A competitive, liquidphase 125 I radioimmunoassay (RIA) was set-up and analytically validated by determination of lower limit of detection (LLOD), dilutional parallelism, spiking recovery, and intra-and inter-assay variability. Surplus canine serum samples were pooled and used for validation. In addition, due to the inability to find canine s.....
Document: Canine specific CD25 protein and antibodies were commercially available and used to establish this assay. A competitive, liquidphase 125 I radioimmunoassay (RIA) was set-up and analytically validated by determination of lower limit of detection (LLOD), dilutional parallelism, spiking recovery, and intra-and inter-assay variability. Surplus canine serum samples were pooled and used for validation. In addition, due to the inability to find canine serum samples with naturally-occurring high concentrations of sCD25, three samples were spiked with different concentrations of pure canine CD25 and treated as unknown samples to test the assay across the entire working range. Recovery was evaluated by spiking three samples with five different concentrations of CD25 (5, 10, 25, 35, and 40 ng/mL).
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