Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_376
Snippet: We evaluated the composition of the fecal microbiota in 24 growing female Beagle dogs 7 times from 5 to 21 months of age by Illumina sequencing and quantitative PCR of the 16S rRNA gene. The population of dogs was homogenous in terms of age, age at neutering (8 months), environmental and feeding conditions. They were fed the same diet ad libitum for 3.5 hours daily. Up to 10.5 months of age, they were fed a puppy growth diet (fat = 20%, total die.....
Document: We evaluated the composition of the fecal microbiota in 24 growing female Beagle dogs 7 times from 5 to 21 months of age by Illumina sequencing and quantitative PCR of the 16S rRNA gene. The population of dogs was homogenous in terms of age, age at neutering (8 months), environmental and feeding conditions. They were fed the same diet ad libitum for 3.5 hours daily. Up to 10.5 months of age, they were fed a puppy growth diet (fat = 20%, total dietary fiber = 8%, crude protein = 29%, on a dry matter basis (DM)) and subsequently an adult maintenance diet (fat = 11%, total dietary fiber = 17%, crude protein = 28% on as DM basis). The dogs were allotted a posteriori into 3 groups according to fat-free mass, fat mass percentage and pelvic circumference at 24 months: optimal body weight (IW, n = 9), moderately overweight (OW1, n = 6) and overweight (OW2, n = 9). The data were analyzed by a linear mixed-effect model with the animal as a random term and corrected for multiple comparisons.
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