2017 ACVIM Forum Research Abstract Program

Fecal , and ursodeoxycholic acid [UDCA]) were measured in their unconjugated form after undergoing butyl esterification for chromatographic separation. A capillary DB-1 ms Ultra Inert column was used with a 20:1 split sample injection ratio. Validation parameters included the lower and upper limits of quantification (LLOQ and ULOQ, respectively). Additionally, precision of the assay was calculated by assaying 6 aliquots taken from a single fecal sample from 4 dogs on the same run/day followed by calculating intraassay coefficients of variation (CV%). Reproducibility of the assay was determined by analyzing 6 aliquots taken from a single fecal sample from 4 dogs on 6 consecutive days followed by calculating inter-assay variation (CV%).