2017 ACVIM Forum Research Abstract Program

The animals for analysis consisted of eight healthy dogs and nine atopy dogs that had never been treated. The diagnosis of CAD was made with fulfillment of clinical criteria (Farvot's criteria) and exclusion of differential diagnosis including ectoparasitic and infectious dermatoses. Expression of Tregs in peripheral blood was evaluated by flow cytometry. Tregs were identified as CD4 + CD25 + PoxP3 + T cells and CD4 + PoxP3 + T cells. There were statistically significant increase in proportion of both CD4 + CD25 + FoxP3 + T cell and CD4 + FoxP3 + T cell in periphery blood of atopic dogs compared to healthy dogs.(mean 0.91% verse 4.2%, P = 0.012; mean 1.76% verse 10.06%, P = 0.004). The circulating regulatory T cells (Tregs) of atopic dogs had negative correlation with their disease severity. (r=À0.758 P = 0.05) and also negative with age (r=À0.666 P = 0.05).