2017 ACVIM Forum Research Abstract Program

Administration of MucosImmune lessened some clinical signs of FHV-1 in both experiments in this model and decreased FHV-1 shedding when administered 24 hours prior to inoculation with FHV-1. Sporotrichosis is the most frequent subcutaneous mycosis detected in urban areas, it is caused by zoonotic pathogenic species of Sporothrix schenckii complex that affects especially cats. In Brazil, due to a large outbreak of sporotrichosis in the city of Guarulhos (State of São Paulo), the disease has become notifiable. Clinically the disease is characterized as a subcutaneous mycosis, with papular lesions with nodular feature or ulcero-gummy. The definitive diagnosis is obtained by the isolation of the fungus in culture. However, the result can take weeks, resulting in a delay to start the appropriate treatment and disease could spread through other community members. The aim of this study was standardization of the diagnosis of feline sporotrichosis by Cell Block Cytology (CB). CB consists of immediate wet cell fixation in preservative liquid based cytology solution (Surepath, BD), and histological processing. We compared two collecting methods: fine needle aspiration (FNA) and exfoliative brushing (EB) with an endocervical brush. Ten samples of skin lesions of nine suspects cats were collected, (five samples with each method). EB allowed a cell thick sediment, and production of several histological sections, with a slide stained with periodic acid Schiff method. All samples collected by this method showed positive results for fungal structures. FNA cases revealed low cellularity and impossible histological processing. This standardization shows that CB processing is possible with EB of lesions and fixation in cytology preservative solution, allowing storage for up to a month after harvest and further testing, as immunohistochemistry. CB is an alternative for sporotrichosis diagnosis. Canine leptospirosis, a disease of worldwide distribution and significant zoonotic potential, is an acute bacterial infection of dogs caused by pathogenic spirochetes belonging to the genus Leptospira. Timely diagnosis of canine leptospirosis is important because early initiation of antibiotic therapy provides the greatest opportunity for a successful patient outcome. However, diagnosis is hindered by vague clinical signs and by diagnostic methods that can be difficult to interpret and require a lag time for results to be reported. A lateral flow test (WITNESS Ò Lepto, Zoetis) has been developed for the detection of IgM antibodies to Leptospira, offering the advantage of a result in 10 minutes at the point-ofcare without the need for specialized equipment or expertise. Our hypothesis is WITNESS Lepto can detect IgM to four significant Leptospira serovars and will yield positive results earlier or concurrent to that of the microscopic agglutination test (MAT), the serological reference method for diagnosis of leptospirosis.