2017 ACVIM Forum Research Abstract Program

Whole blood impedance platelet aggregometry and tissue factor-activated thromboelastography (TF-TEG) were performed in six healthy client-owned Greyhounds and six healthy laboratory Beagles. For platelet aggregometry, the area under the curve was recorded for each agonist (adenosine diphosphate (ADP) and arachidonic acid (AA)) and a saline control. For TF-TEG, reaction time (R), clotting time (K), rate of clot formation (a), maximum amplitude (MA), percent clot lysis 30 minutes after MA is reached (LY30), amount of clot lysis 30 minutes after MA is reached (CL30), percent clot lysis 60 minutes after the MA is reached (LY60), and amount of clot lysis 60 minutes after MA is reached (CL60) were recorded. The hematocrit, platelet count, and fibrinogen were also recorded. An unpaired t-test was performed on ADP, AA, hematocrit, platelet count, fibrinogen, R, a, MA, and a Mann-Whitney U test was performed on the saline control, K, LY30, CL30, LY60, and CL60. Pearson correlation was also assessed between hematocrit and ADP, AA, and MA, platelet count and ADP, AA, and MA, and fibrinogen and MA. For all tests, P < 0.05 was considered significant. The ADP, AA, platelet count, fibrinogen, MA, CL30, and CL60 were significantly lower and the hematocrit, LY30, and LY60 were significantly higher in the Greyhounds. There were no statistical differences for the saline control, R, K, and a between groups. In all twelve dogs, there was correlation between hematocrit and MA (r = À0.70), ADP (r = À0.91), and AA (r = À0.81), and platelet count and ADP (r = 0.9), AA (r = 0.72), and MA (r = 0.68) and fibrinogen and MA (r = 0.85).