Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_576
Snippet: The authors obtained early morning urine samples from nine healthy bitches. Following urine filtration through a 0.2 lm biofilter, we obtained a matrix of three levels of pH (5.5, 7.0, 8.5) by three levels of urine specific gravity (USG; 1.010, 1.020, 1.030) by titrating the urine with HCl and NaOH. We inoculated the urine with known quantities of three UPEC strains, incubated at 37°C for four hours, made serial dilutions from 10 À4 to 10 À7 ,.....
Document: The authors obtained early morning urine samples from nine healthy bitches. Following urine filtration through a 0.2 lm biofilter, we obtained a matrix of three levels of pH (5.5, 7.0, 8.5) by three levels of urine specific gravity (USG; 1.010, 1.020, 1.030) by titrating the urine with HCl and NaOH. We inoculated the urine with known quantities of three UPEC strains, incubated at 37°C for four hours, made serial dilutions from 10 À4 to 10 À7 , plated on nutrient agar for 24 hours and subsequently counted the numbers of colonies forming units (CFU). We only counted plates from dilutions that grew 30-300 CFU. All experiments had negative and positive controls.
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