2017 ACVIM Forum Research Abstract Program

In the IN model, serum, urine, peripheral blood mononuclear cells, and a renal biopsy were collected from six, one year old, healthy purpose bred, unvaccinated kittens (three male; three female). After sample collection on Week 0 and again on Weeks 2, 4, 6, 8, 10, 12, and 14, all cats were administered a commercially available parenteral FVRCP vaccine shown previously to induce anti-CRFK and anti-enolase antibodies. A second renal biopsy was collected on Week 16, avoiding the previous biopsy sites. Blood and urine samples were collected on all sample dates. A single lymphocyte proliferation assay was performed on 4 of 6 hyperinoculated cats and 2 unvaccinated healthy control cats using 4 antigens (kidney, spleen, alpha-enolase, and CRFK). Sera were assayed using a commercially available kit that detects sFas, Flt-3 ligand, GM-CSF, IFN-gamma, IL-1beta, IL-2, IL-4, SDF-1, KC, IL-6, MCP-1, IL-13, IL-8, IL-12 (p40), PDGF-BB, RANTES, SCF, IL-18, and TNF-alpha (Milliplex MAP Cytokine/Chemokine Feline Panel, Millipore, Darmstadt, Germany). Alpha-enolase immunohistochemical staining was performed on kidney biopsy samples (week 0 and week 16) and then evaluated and scored (0-4) by a board-certified pathologist that was masked to the timing of the biopsies.