2017 ACVIM Forum Research Abstract Program

Twenty-one healthy client-owned dogs were enrolled. First, in vitro IC50 testing was performed twice (trial 1 and 2) at least 1 week apart to determine individual repeatability. Whole blood samples were drawn and incubated with 6 different aspirin concentrations. Aggregation was measured in AUC (area under the curve) units using a multiple electrode impedance aggregometer (Multiplate). For in vivo IC50 testing, dogs were given a single oral dose of 4 mg/kg aspirin. Aggregation was assessed prior to aspirin administration then 20, 40, and 180 minutes after administration. At each time point, samples were also collected into EDTA and EDTAfluoride blood tubes for measurement of ASA and SA plasma concentrations using liquid chromatography with mass spectrometry.