Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_710
Snippet: Six healthy adult dogs were given cyclosporine (10 mg/kg BID, PO) for 7 days. Whole blood gene expression of IL-2 and IFN-c was evaluated before treatment and again on Day 7 of drug administration, two hours after giving the medication, using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Samples were activated for 5 hours with phorbol 12-myristate 13-acetate and ionomycin, RNA was extracted, and relative gene quantificat.....
Document: Six healthy adult dogs were given cyclosporine (10 mg/kg BID, PO) for 7 days. Whole blood gene expression of IL-2 and IFN-c was evaluated before treatment and again on Day 7 of drug administration, two hours after giving the medication, using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Samples were activated for 5 hours with phorbol 12-myristate 13-acetate and ionomycin, RNA was extracted, and relative gene quantification was performed comparing gene expression after drug administration to pre-treatment levels. Additionally, for one week after cessation of cyclosporine, daily blood samples were collected for evaluation of IL-2 and IFN-c gene expression.
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