Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_413
Snippet: A blood unit was collected from 6 healthy dogs, processed, then divided into two equal subunits of RBCs, one of which was leukoreduced with a commercial filter. Units were stored at 4°C for 42 days and sampled biweekly. NET markers citrullinated histone H3 and cell-free DNA were quantified in unit supernatants via western blot and fluorescence with DNA dye, respectively. Supernatants were applied ex vivo to neutrophils from healthy dogs and rele.....
Document: A blood unit was collected from 6 healthy dogs, processed, then divided into two equal subunits of RBCs, one of which was leukoreduced with a commercial filter. Units were stored at 4°C for 42 days and sampled biweekly. NET markers citrullinated histone H3 and cell-free DNA were quantified in unit supernatants via western blot and fluorescence with DNA dye, respectively. Supernatants were applied ex vivo to neutrophils from healthy dogs and release of extracellular DNA representing NETosis was assessed. Data was analyzed by repeated measures ANOVA with treatment and time as fixed variables.
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