2017 ACVIM Forum Research Abstract Program

There were significant differences between the concentrations of SDMA and NGAL measured before and measured after the correction of dehydration in dogs with azotemia, but not in dogs without azotemia. In addition, SDMA and NGAL concentrations showed significant decreases after the correction of dehydration in dogs with moderate or severe dehydration, but not in dogs with mild dehydration. Notably, in 16 of 69 dogs, serum SDMA concentrations before correction of dehydration were higher than the reference interval and were normalized to the reference interval after rehydration. The correlation between serum SDMA and creatinine concentrations was significant, regardless of the presence of azotemia; whereas the serum NGAL concentration was significantly correlated with creatinine concentration only in dogs with azotemia. This study found that the circulating SDMA concentration can be affected by hydration status, indicating that measurement of SDMA concentration in dehydrated dogs might need to be repeated after correction of dehydration. However, further studies are needed to clarify our results. Oxidative stress is central to the pathogenesis of many systemic diseases. In humans, antioxidant depletion and lipid peroxidation correlate with disease severity and outcome. We have previously shown ill dogs experience oxidative stress, including decreased glutathione and vitamin E, and increased urinary 8-isoprostane/creatinine (8-IsoP/Cr). Short-term N-acetylcysteine supplementation did not impact clinical score or outcome but prevented further glutathione depletion. The purpose of this study was to evaluate longer-term, combination antioxidant supplementation on markers of oxidative stress, clinical score, and survival in ill dogs.