Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_477
Snippet: Hepatic tissue was collected from 8 healthy dogs during ovariohysterectomy and also from 8 dogs with histologically confirmed chronic hepatitis. The proteome of the liver tissue was extracted by mechanical disruption and detergent based cell lysis and was analyzed by 2-dimensional fluorescence difference gel electrophoresis. Proteins were separated in the first dimension by isoelectric focusing on a 3 -10 nonlinear immobilized pH gradient and by .....
Document: Hepatic tissue was collected from 8 healthy dogs during ovariohysterectomy and also from 8 dogs with histologically confirmed chronic hepatitis. The proteome of the liver tissue was extracted by mechanical disruption and detergent based cell lysis and was analyzed by 2-dimensional fluorescence difference gel electrophoresis. Proteins were separated in the first dimension by isoelectric focusing on a 3 -10 nonlinear immobilized pH gradient and by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. DeCyder 2-D Differential Analysis Software Ã’ was utilized for data analysis. Proteins with an absolute fold change value > 2.0 were selected for further analysis. Protein identification was achieved by nanoflow liquid chromatography tandem mass spectrometry (NANO LC-MS/MS). Post-hoc testing was performed using Bonferroni's multiple comparisons test and significance was set at P < 0.05.
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