2017 ACVIM Forum Research Abstract Program

T lymphocyte proliferative responses to mitogen activation in the dogs on Week 12 (Range = 2.12 -3.9; median = 2.25) was statistically greater (P = 0.03) than Week 0 (1.47 -2.47; median = 1.92). Compared to Week 0, statistically significant increases in B cells expressing surface bound IgG or MHCII and increased B cell MHCII GMFI were first noted on Week 4 and were maintained through Week 12. Compared to Week 0, numerically increased percentages of CD4 + T cells expressing MHCII, percentages of CD8 T cells expressing MHCII, increased T cell CD4 + MHC Class2 GMFI, and increased T cell CD8 + MHCII GMFI were detected Weeks 4, 8 and 12, but the differences were not statistically significant. Compared to Week 0, T cell CD8 + CD11a+ and T cell CD8 + CD11a+ GMFI were significantly greater on Weeks 4 and 8 but the effect was not apparent on Week 12. Of the cytokines/chemokines, at least 50% of the sera assessed had concentrations within the range of the assay for KC-like, IP-10, IL-7, IL-8, IL-18, and MCP-1; statistically significant differences after supplementation with the probiotic were not noted.