2017 ACVIM Forum Research Abstract Program

The objectives of our study were to, utilizing a dose escalation study in healthy dogs, determine the maximum tolerated dose (MTD) of MMF in each animal, and determine the effects of this dose on pharmacodynamic assays (B and T cell proliferation) focused on the established drug mechanism of action. Eight adult hound dogs were given oral MMF at three different escalating dose rates (10 mg/kg bid, 15 mg/kg bid, and 20 mg/kg bid) for one week at each dose, with a 2 week washout period between doses, and monitored for significant diarrhea, vomiting or inappetence. Once MTD was determined, dogs were administered this dose rate for 2 weeks, and blood was collected on Days 0, 7 and 14 for B and T cell proliferation assays measuring incorporation of tritiated thymidine following activation with pokeweed mitogen and concanavalin A, respectively. A complete blood count (CBC), serum biochemistry, and urinalysis were also performed at the beginning and end of the study.