A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons

Liu, Xingjian; Yang, Xin; Mehboob, Arslan; Hu, Xiaoyuan; Yi, Yongzhu; Li, Yinü; Zhang, Zhifang

According to published procedures (Liu et al., 2016) , the pVL1393-Cα or pVL1393-Cγ vector was mixed with the reBmBac vector. The mixture was used to cotransfect Bm5 cells. chIFN-α and chIFN-γ genes were inserted into the baculovirus genome at the polyhedron gene site. The recombinant baculoviruses were named reBm-Cα and reBm-Cγ. A mixture of the pVL1393-Cα vector and reBmBac-p10Cγ was then utilized to cotransfect Bm5 cells. chIFN-α was transferred into the reBmBac-p10Cγ genome at the polyhedron gene site. The recombinant baculovirus, which contained the chIFN-α gene at the polyhedron site and the chIFN-γ gene at the p10 site, was named reBm-Cαγ. The recombinant baculoviruses reBm-Cαγ, reBm-Cα, and reBm-Cγ were purified by plaque screening (Pen, Welling, & Welling-Wester, 1989 ).